Document 0801
 DOCN  M94B0801
 TI    Inhibition of herpes simplex virus 1 reproduction with hydrophobized
       antisense oligonucleotides.
 DT    9412
 AU    Vinogradov SV; Suzdaltseva Y; Alakhov VYu; Kabanov AV; Moscow Institute
       of Biotechnology Inc., Russia.
 SO    Biochem Biophys Res Commun. 1994 Sep 15;203(2):959-66. Unique Identifier
       : AIDSLINE MED/94380082
 AB    Antisense oligonucleotides were modified by their ends with hydrophobic
       substituents which permitted enhancing their activity. The effect of
       hydrophobized oligonucleotides on reproduction of Herpes Simplex Virus
       type 1 in Vero cells was studied. Two types of oligonucleotides were
       used: a 12-mer complementary to the splicing site 983-994 of early
       mRNA-5 of the virus and 19-mer complementary to the site of mRNA that
       encodes the virus DNA-polymerase. These oligonucleotides were modified
       by 5'-ends with n-undecyl or cholesteryl moieties and by 3'-ends with
       acridine or 1,7-heptanediol. In comparison with the unmodified
       oligonucleotides the hydrophobized ones were significantly more active
       and inhibited HSV-1 infection at micromolar concentrations.
 DE    Acridines/CHEMISTRY  Animal  Base Sequence  Cholesterol/CHEMISTRY  DNA
       Polymerases/GENETICS  Glycols/CHEMISTRY  HIV-1/DRUG EFFECTS/*GROWTH &
       DEVELOPMENT  Molecular Sequence Data  Oligonucleotides,
       Antisense/CHEMISTRY/*PHARMACOLOGY  RNA Splicing  RNA, Messenger  RNA,
       Viral  Vero Cells  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).